Journal: iScience

Article Title: PAR2-β-arrestin 2-ERK axis mediates Malassezia globosa -induced IL-17 response by disrupting ZO-1 in keratinocytes

doi: 10.1016/j.isci.2026.115646

Figure Lengend Snippet: Impact of M. globosa infection on PAR2 activation, IL-17 production, and TJ integrity in HaCaT cells (A) Cell survival rate was detected by CCK8 assay. (B–D) Protein expression and correlation analysis in HaCaT cells infected with 30 MOI M. globosa was evaluated by western blot at various time. (E and F) mRNA levels and correlation analysis were measured by reverse transcription quantitative PCR (RT-qPCR). Red indicated upregulated gene expression, green indicated downregulated gene expression. (G) IL-17A levels were detected by ELISA test in the supernatant of HaCaT cells infected with 30 MOI M. globosa . Red indicated upregulated gene expression (C and E) or positive correlation (D and F), blue indicated downregulated gene expression (C and E) or negative correlation (D and F).These experiments (A, B, E, and G) were representative of 3 independent experiments. Values are means ± SD. a two-way ANOVA with Bonferroni’s (A), or Dunnet’s (G) multiple comparisons test was applied. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Correlation analysis is performed using Pearson’s correlation coefficient analysis. See also .

Article Snippet: Cell viability was determined with the Cell Counting Kit-8 (CCK8, Beyotime, Shanghai, China), following the manufacturer’s protocol.

Techniques: Infection, Activation Assay, CCK-8 Assay, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay

Journal: Cancer Medicine

Article Title: Artesunate Induces G0 / G1 Phase Arrest in Tumor Cells and Associates With Cyclin‐Dependent Kinase 4 ( CDK4 )

doi: 10.1002/cam4.71907

Figure Lengend Snippet: Chemical structures and equipotent antiproliferative activity of artesunate and the artesunate–biotin probe. (A) Chemical structures of artesunate and the artesunate–biotin probe. (B) Comparison of the antiproliferative effects of artesunate and the artesunate–biotin probe against NSCLC cells after 72 h of treatment (CCK8 assay, n = 3; mean ± SD).

Article Snippet: Cell Counting Kit‐8 (CCK8) was from DOJINDO (Shanghai, China).

Techniques: Activity Assay, Comparison, CCK-8 Assay